Function of miR-24 and miR-27 in Pediatric Patients With Idiopathic Nephrotic Syndrome

Purpose: We investigated the pathogenesis of idiopathic nephrotic syndrome (INS) by measuring effects two specific miRNAs on Th2 cells in children with this disease. Methods: After informed consent, we enrolled 20 active INS before steroid initiation, remission after therapy, and age-matched healthy controls. Flow cytometry was used to measure levels a cytometric bead array IgE, interleukin (IL)?4, IL-13. RT-PCR miR-24 miR-27 CD4 + TCD25 ? cells. PBMCs were isolated using Ficoll density gradient centrifugation, transfected different mimic or inhibitor miRNAs. expression RNAs, flow determine percentage Results: Relative controls, had higher percentages ( P < 0.05), but there no significant difference controls remission. The plasma IL-4, IL-13 significantly increased 0.05). There lower non-atopic Transfection experiments indicated that upregulation each miRNA decreased level IL-4 down-regulation opposite Conclusion: Children INS, without atopy, possibly related their due drift toward suppressed have critical function regulating cell INS.